Abstract :
Enhanced features can be easily segmented and used for classification algorithms. Light wavelength of
510nm is used as illumination source for bright field microscope. This specific wavelength of light enhances
nucleus, granules in White blood Cells (WBCs). Eosinophil and basophil has coarse granules which also
get enhanced hence nucleus segmentation gets difficult. This method is effective in enhancing nucleus of
agranulocytes viz. Lymphocyte, Monocyte and Neutrophil of normal person. This modification in image
acquisition of pathological images will further help in quick segmentation of features.
Keyword :
Bright field microscope, Image enhancement, 510nm wavelength of green light, LED illumination, WBC nucleus.